In this fully revised edition of an established classic, expert researchers and clinicians describe in step-by-step detail updated techniques for the isolation and growth of the major primary cell types, such as kidney proximal tubule cells, hepatocytes, keratinocytes, and cardiomyocytes. The authors offer readily reproducible new methods for the differentiation of embryonic stem (ES) cells into various hematopoietic cell types, for fetal thymic organ culture, and for the isolation and culture of specialized cell types, such as mammary progenitor cells, skeletal muscle myofibers, mesenchymal cells, neural stem cells, hematopoietic cells, stromal cell lines, and endothelial cells. Additional chapters describe new techniques (leukocyte rolling, isolation of side population cells, and scalable production of ES-derived cells) and detail quality control methods for cell lines (detection and elimination of mycoplasma, DNA fingerprinting, and cytogenetic analysis). The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Up-to-date and highly practical, Basic Cell Culture Protocols, Third Edition, offers basic scientists and clinician-researchers powerful tools to isolate, culture, and characterize the promising specialized cell types in demand today.

在这本经典著作的全面修订版中，专家研究人员和临床医生逐步详细描述了主要原始细胞类型的分离和生长的最新技术，如肾脏近端小管细胞、肝细胞、角质细胞和心肌细胞。作者提供了易于复制的新方法，用于将胚胎干（ES）细胞分化为各种造血细胞类型，用于胎儿胸腺器官培养，以及用于分离和培养特殊细胞类型，如乳腺祖细胞、骨骼肌肌纤维、间质细胞、神经干细胞、造血细胞、基质细胞系和内皮细胞。其他章节描述了新的技术（白细胞滚动，分离边群细胞，以及ES衍生细胞的可扩展生产），并详细介绍了细胞系的质量控制方法（检测和消除支原体，DNA指纹，和细胞遗传学分析）。这些协议遵循成功的《分子生物学方法》系列格式，每个协议都提供逐步的实验室说明、概述技术背后原理的介绍、必要的设备和试剂清单，以及关于故障排除和避免已知陷阱的提示。

基础细胞培养协议》第三版是最新的、高度实用的，它为基础科学家和临床研究人员提供了强大的工具，以分离、培养和描述当今有前途的特殊细胞类型。